Questioning the reliability of estimates of enzyme inhibitor constant: Case of competitive inhibition
نویسنده
چکیده
Reliability of kinetic parameters are crucial in understanding enzyme kinetics within cellular system. The present study suggests a few cautions that need introspection for estimation of parameters like M K , Max V and I K using Lineweaver-Burk plots. The quality of 50 IC too needs a thorough reinvestigation because of its direct link with I K and M K values. Inhibition kinetics under both steady-state and non-steady-state conditions are studied and errors in estimated parameters are compared against actual values to settle the question of their adequacy.
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